kerafast货号EVU102组学UV Cleavable Biotin-Azide

The UV Cleavable Biotin-Azide allows the tagging of alkyne modified substrates via copper-catalyzed click chemistry.

Highlights:

• Biotin conjugate can be subse visualized by streptavidin Western techniques and/or enriched using streptavidin purification methods

• Captured target can be released under mild photolysis conditions (365 nm) and results in a significant decrease of background signal due to non-specifically bound proteins

• Also available: 13C6-UV-Biotin-azide

产品参数：


反应原理示意图：

参考文献：
1. Alkylation Damage by Lipid Electrophiles Targets Functional Protein Systems. Codreanu, S. G.; Ullery, J. C.; Zhu, J.; Tallman, K. A.; Beavers, W. N.; Porter, N. A.; Marnett, L. J.; Zhang, B.; Liebler, D. C. Mol. Cell. Proteomics 2014, 13, 849.
2. An Azido-Biotin Reagent for Use in the Isolation of Protein Adducts of Lipid-Derived Electrophiles by Streptavidin Catch and Photo-Release. Kim, H.-Y. H.; Tallman, K. A.; Liebler, D. C.; Porter, N. A. Mol. Cell. Proteomics 2009, 8, 2080.
3. Yang J, Gupta V, Tallman KA, Porter NA, Carroll KS, Liebler DC. Global, in situ, site-specific analysis of protein S-sulfenylation. Nat Protoc. 2015 Jul;10(7):1022-37. View Article
4. Fu L, Liu K, Ferreira RB, Carroll KS, Yang J. Proteome-Wide Analysis of Cysteine S-Sulfenylation Using a Benzothiazine-Based Probe. Curr Protoc Protein Sci. 2019 Feb;95(1):e76. View Article